IlvB
- Description: acetolactate synthase (large subunit)
Gene name | ilvB |
Synonyms | |
Essential | no |
Product | acetolactate synthase (large subunit) |
Function | biosynthesis of branched-chain amino acids |
Metabolic function and regulation of this protein in SubtiPathways: Ile, Leu, Val, Coenzyme A | |
MW, pI | 62 kDa, 5.178 |
Gene length, protein length | 1722 bp, 574 aa |
Immediate neighbours | ilvH, ysnD |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU28310
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids
This gene is a member of the following regulons
CodY regulon, CcpA regulon, TnrA regulon, T-box
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 2 pyruvate = 2-acetolactate + CO2 (according to Swiss-Prot)
- Protein family: TPP enzyme family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure: 1N53 (T box RNA)
- UniProt: P37251
- KEGG entry: [3]
- E.C. number: 2.2.1.6
Additional information
- subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and regulation
- Regulation: for a complete overview on the regulation of the ilv operon, see Brinsmade et al.
- Regulatory mechanism:
- Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed
Biological materials
- Mutant: GP324, ilvB under control of pXyl (cat), available in Jörg Stülke lab
- Expression vector:
- lacZ fusion: pGP520 and pGP524 (both in pAC5), available in Stülke lab; pGP523 (in pAC6), available in Stülke lab
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed