ProI

• Description: pyrroline-5-carboxylate reductase
  
  

Gene name	proI
Synonyms	yqjO
Essential	no
Product	pyrroline-5-carboxylate reductase
Function	biosynthesis of proline
Metabolic function and regulation of this protein in SubtiPathways: Pro, Stress
MW, pI	30 kDa, 6.702
Gene length, protein length	834 bp, 278 aa
Immediate neighbours	yqjP, yqjN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU23800

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-proline + NAD(P)⁺ = 1-pyrroline-5-carboxylate + NAD(P)H (according to Swiss-Prot)

• Protein family: pyrroline-5-carboxylate reductase family (according to Swiss-Prot)

• Paralogous protein(s): ProH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P54552

• KEGG entry: [3]

• E.C. number: 1.5.1.2

Expression and regulation

• Operon: proI (according to DBTBS)

• Sigma factor: SigA PubMed

• Regulation:
  • induced by proline limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jeanette Brill, Tamara Hoffmann, Harald Putzer, Erhard Bremer
T-box-mediated control of the anabolic proline biosynthetic genes of Bacillus subtilis.
Microbiology (Reading): 2011, 157(Pt 4);977-987
[PubMed:21233158] [WorldCat.org] [DOI] (I p)

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

B R Belitsky, J Brill, E Bremer, A L Sonenshein
Multiple genes for the last step of proline biosynthesis in Bacillus subtilis.
J Bacteriol: 2001, 183(14);4389-92
[PubMed:11418582] [WorldCat.org] [DOI] (P p)