GalK

• Description: galactokinase
  
  

Gene name	galK
Synonyms	ipa-35d
Essential	no
Product	galactokinase
Function	galactose utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	43 kDa, 5.148
Gene length, protein length	1170 bp, 390 aa
Immediate neighbours	galT, gtaC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

• Locus tag: BSU38200

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + D-galactose = ADP + alpha-D-galactose 1-phosphate (according to Swiss-Prot)

• Protein family: GalK subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1PIE (from Lactococcus lactis, 43% identity, 60% similarity) PubMed

• UniProt: P39574

• KEGG entry: [3]

• E.C. number: 2.7.1.6

Expression and regulation

• Operon: galK-galT PubMed

• Sigma factor:

• Regulation:
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Y Miwa, A Nakata, A Ogiwara, M Yamamoto, Y Fujita
Evaluation and characterization of catabolite-responsive elements (cre) of Bacillus subtilis.
Nucleic Acids Res: 2000, 28(5);1206-10
[PubMed:10666464] [WorldCat.org] [DOI] (I p)

E Presecan, I Moszer, L Boursier, H Cruz Ramos, V de la Fuente, M-F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-3328
[PubMed:9353933] [WorldCat.org] [DOI] (P p)