GndA

• Description: NADP-dependent phosphogluconate dehydrogenase
  
  

Gene name	gndA
Synonyms	yqjI
Essential	no
Product	NADP-dependent phosphogluconate dehydrogenase
Function	pentose phosphate pathway
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	51 kDa, 5.076
Gene length, protein length	1407 bp, 469 aa
Immediate neighbours	zwf, polY1
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

carbon core metabolism, phosphoproteins

The gene

Basic information

• Locus tag: BSU23860

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 6-phospho-D-gluconate + NADP⁺ = D-ribulose 5-phosphate + CO₂ + NADPH (according to Swiss-Prot)

• Protein family: 6-phosphogluconate dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: Reversible reaction PubMed

• Domains:

• Modification: phosphorylation on (Thr-15 OR Thr-17) PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2W8Z (from Geobacillus stearothermophilus, 83% identity, 92% similarity) PubMed

• UniProt: P80859

• KEGG entry: [3]

• E.C. number: 1.1.1.44

Additional information

It contains a cysteine on the active site. The enzyme is a dimer. PubMed

Expression and regulation

• Operon: polY1-gndA (according to DBTBS)

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Nicola Zamboni, Eliane Fischer, Dietmar Laudert, Stéphane Aymerich, Hans-Peter Hohmann, Uwe Sauer
The Bacillus subtilis yqjI gene encodes the NADP+-dependent 6-P-gluconate dehydrogenase in the pentose phosphate pathway.
J Bacteriol: 2004, 186(14);4528-34
[PubMed:15231785] [WorldCat.org] [DOI] (P p)