Ddl

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  • Description: D-alanine-D-alanine ligase

Gene name ddl
Synonyms ddlA
Essential yes PubMed
Product D-alanine-D-alanine ligase
Function peptidoglycan precursor biosynthesis
Metabolic function and regulation of this protein in SubtiPathways:
Cell wall
MW, pI 38 kDa, 4.58
Gene length, protein length 1062 bp, 354 aa
Immediate neighbours ydbP, murF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Ddl context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, cell envelope stress proteins (controlled by SigM, W, X, Y), essential genes

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

  • Locus tag: BSU04560

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine (according to Swiss-Prot)
  • Protein family: ATP-grasp domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure: 2YZG (from Thermus thermophilus hb8 mutant, 40% identity, 53% similarity)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)