AzoR1

• Description: azoreductase, involved in quinone detoxification
  
  

Gene name	azoR1
Synonyms	yocJ
Essential	no
Product	azoreductase
Function	quinone detoxification
MW, pI	22 kDa, 4.994
Gene length, protein length	624 bp, 208 aa
Immediate neighbours	bsrB, yocK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU19230

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

resistance against oxidative and electrophile stress

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): AzoR2

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3P0R (from B. anthracis, 64% identity, 81% similarity)

• UniProt: O35022

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • induced by thiol-reactive compounds (catechol, menaquinone) (YodB) PubMed

• Regulatory mechanism:
  • YodB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Montira Leelakriangsak, Nguyen Thi Thu Huyen, Stefanie Töwe, Nguyen van Duy, Dörte Becher, Michael Hecker, Haike Antelmann, Peter Zuber
Regulation of quinone detoxification by the thiol stress sensing DUF24/MarR-like repressor, YodB in Bacillus subtilis.
Mol Microbiol: 2008, 67(5);1108-24
[PubMed:18208493] [WorldCat.org] [DOI] (I p)

Stefanie Töwe, Montira Leelakriangsak, Kazuo Kobayashi, Nguyen Van Duy, Michael Hecker, Peter Zuber, Haike Antelmann
The MarR-type repressor MhqR (YkvE) regulates multiple dioxygenases/glyoxalases and an azoreductase which confer resistance to 2-methylhydroquinone and catechol in Bacillus subtilis.
Mol Microbiol: 2007, 66(1);40-54
[PubMed:17725564] [WorldCat.org] [DOI] (P p)