YisZ

• Description: adenylylsulfate kinase
  
  

Gene name	yisZ
Synonyms
Essential	no
Product	adenylylsulfate kinase
Function	sulfate reduction and activation
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	22 kDa, 7.211
Gene length, protein length	597 bp, 199 aa
Immediate neighbours	yisY, yitA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU10910

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

sulfur metabolism, sporulation proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate (according to Swiss-Prot)

• Protein family: APS kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3CR8 (from Escherichia coli, 38% identity, 55% similarity) PubMed

• UniProt: O06735

• KEGG entry: [3]

• E.C. number: 2.7.1.25

Expression and regulation

• Operon: yitB-yitA-yisZ (according to DBTBS)

• Sigma factor: SigK PubMed

• Regulation:
  • expressed during sporulation in the mother cell (SigK) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)