TcyA

• Description: cystine ABC transporter (binding protein)
  
  

Gene name	tcyA
Synonyms	yckK
Essential	no
Product	cystine ABC transporter (binding protein)
Function	cystine uptake
MW, pI	29 kDa, 9.118
Gene length, protein length	804 bp, 268 aa
Immediate neighbours	tcyB, bsdA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU03610

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

ABC transporters, biosynthesis/ acquisition of amino acids, membrane proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: bacterial solute-binding protein 3 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: TcyC-TcyB-TcyA PubMed

• Localization: attached to the membrane via TcyB PubMed

Database entries

• Structure:

• UniProt: P42199

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: tcyA-tcyB-tcyC (putative) PubMed

• Sigma factor:

• Regulation: very weakly expressed under all conditions PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Pierre Burguière, Sandrine Auger, Marie-Françoise Hullo, Antoine Danchin, Isabelle Martin-Verstraete
Three different systems participate in L-cystine uptake in Bacillus subtilis.
J Bacteriol: 2004, 186(15);4875-84
[PubMed:15262924] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

F Rodriguez, G Grandi
An operon encoding a novel ABC-type transport system in Bacillus subtilis.
Microbiology (Reading): 1995, 141 ( Pt 7);1781-4
[PubMed:7551042] [WorldCat.org] [DOI] (P p)