StoA

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  • Description: thiol-disulfide oxidoreductase, with thioredoxin-like domain, required for the synthesis of the endospore peptidoglycan cortex

Gene name stoA
Synonyms spoIVH, ykvV
Essential no
Product thiol-disulfide oxidoreductase
Function spore cortex formation
MW, pI 18 kDa, 5.297
Gene length, protein length 495 bp, 165 aa
Immediate neighbours ykvU, zosA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YkvV context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU13840

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

Categories containing this gene/protein

sporulation proteins

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: reduction of intramolecular disulfide bonds in SpoVD PubMed
  • Protein family: thioredoxin domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: forespore envelope PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Lars Hederstedt, University of Lund, Sweden Homepage

Your additional remarks

References

Reviews

Patrick Eichenberger
The red-ox status of a penicillin-binding protein is an on/off switch for spore peptidoglycan synthesis in Bacillus subtilis.
Mol Microbiol: 2010, 75(1);10-2
[PubMed:19919674] [WorldCat.org] [DOI] (I p)

Original Publications

Yiming Liu, Mirja Carlsson Möller, Lise Petersen, Christopher A G Söderberg, Lars Hederstedt
Penicillin-binding protein SpoVD disulphide is a target for StoA in Bacillus subtilis forespores.
Mol Microbiol: 2010, 75(1);46-60
[PubMed:19919673] [WorldCat.org] [DOI] (I p)

Allister Crow, Yiming Liu, Mirja Carlsson Möller, Nick E Le Brun, Lars Hederstedt
Structure and functional properties of Bacillus subtilis endospore biogenesis factor StoA.
J Biol Chem: 2009, 284(15);10056-66
[PubMed:19144642] [WorldCat.org] [DOI] (P p)

Lyethur S Erlendsson, Mirja Möller, Lars Hederstedt
Bacillus subtilis StoA Is a thiol-disulfide oxidoreductase important for spore cortex synthesis.
J Bacteriol: 2004, 186(18);6230-8
[PubMed:15342593] [WorldCat.org] [DOI] (P p)

Daisuke Imamura, Kazuo Kobayashi, Junichi Sekiguchi, Naotake Ogasawara, Michio Takeuchi, Tsutomu Sato
spoIVH (ykvV), a requisite cortex formation gene, is expressed in both sporulating compartments of Bacillus subtilis.
J Bacteriol: 2004, 186(16);5450-9
[PubMed:15292147] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)