PbuE

• Description: putative hypoxanthine exporter
  
  

Gene name	pbuE
Synonyms	ydhL
Essential	no
Product	putative hypoxanthine exporter
Function	nucleotide metabolism
MW, pI	41 kDa, 8.884
Gene length, protein length	1164 bp, 388 aa
Immediate neighbours	ydhK, gmuB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU05800

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

transporters/ other, nucleotide metabolism/ other, membrane proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: Q797E3

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • induced by hypoxanthine and guanine, PurR-independent PubMed
  • induced by adenine (A-box) PubMed

• Regulatory mechanism:
  • A-box: adenine-controlled riboswitch, transcription termination/ antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Vanessa Delfosse, Patricia Bouchard, Eric Bonneau, Pierre Dagenais, Jean-François Lemay, Daniel A Lafontaine, Pascale Legault
Riboswitch structure: an internal residue mimicking the purine ligand.
Nucleic Acids Res: 2010, 38(6);2057-68
[PubMed:20022916] [WorldCat.org] [DOI] (I p)

Elias Seif, Sidney Altman
RNase P cleaves the adenine riboswitch and stabilizes pbuE mRNA in Bacillus subtilis.
RNA: 2008, 14(6);1237-43
[PubMed:18441052] [WorldCat.org] [DOI] (I p)

Natalia P Zakataeva, Sergey V Gronskiy, Anastasia S Sheremet, Ekaterina A Kutukova, Anna E Novikova, Vitaliy A Livshits
A new function for the Bacillus PbuE purine base efflux pump: efflux of purine nucleosides.
Res Microbiol: 2007, 158(8-9);659-65
[PubMed:17935948] [WorldCat.org] [DOI] (P p)

K V Lobanov, N V Korol'kova, S Iu Eremina, L Errais Lopes, S A Proshkin, A S Mironov
[Mutations altering the specificity of the sensor RNA encoded by the Bacillus subtilis pbuE gene].
Genetika: 2007, 43(6);859-64
[PubMed:17853814] [WorldCat.org] (P p)

Jean-François Lemay, J Carlos Penedo, Renaud Tremblay, David M J Lilley, Daniel A Lafontaine
Folding of the adenine riboswitch.
Chem Biol: 2006, 13(8);857-68
[PubMed:16931335] [WorldCat.org] [DOI] (P p)

J Kenneth Wickiser, Ming T Cheah, Ronald R Breaker, Donald M Crothers
The kinetics of ligand binding by an adenine-sensing riboswitch.
Biochemistry: 2005, 44(40);13404-14
[PubMed:16201765] [WorldCat.org] [DOI] (P p)

Per Nygaard, Hans H Saxild
The purine efflux pump PbuE in Bacillus subtilis modulates expression of the PurR and G-box (XptR) regulons by adjusting the purine base pool size.
J Bacteriol: 2005, 187(2);791-4
[PubMed:15629952] [WorldCat.org] [DOI] (P p)

Maumita Mandal, Ronald R Breaker
Adenine riboswitches and gene activation by disruption of a transcription terminator.
Nat Struct Mol Biol: 2004, 11(1);29-35
[PubMed:14718920] [WorldCat.org] [DOI] (P p)

Lars Engholm Johansen, Per Nygaard, Catharina Lassen, Yvonne Agersø, Hans H Saxild
Definition of a second Bacillus subtilis pur regulon comprising the pur and xpt-pbuX operons plus pbuG, nupG (yxjA), and pbuE (ydhL).
J Bacteriol: 2003, 185(17);5200-9
[PubMed:12923093] [WorldCat.org] [DOI] (P p)

Maumita Mandal, Benjamin Boese, Jeffrey E Barrick, Wade C Winkler, Ronald R Breaker
Riboswitches control fundamental biochemical pathways in Bacillus subtilis and other bacteria.
Cell: 2003, 113(5);577-86
[PubMed:12787499] [WorldCat.org] [DOI] (P p)