OhrA

• Description: peroxidase, protects the cell against organic peroxides
  
  

Gene name	ohrA
Synonyms	yklA
Essential	no
Product	peroxidase
Function	organic peroxide resistance
MW, pI	14 kDa, 5.061
Gene length, protein length	423 bp, 141 aa
Immediate neighbours	proA, ohrR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU13140

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

resistance against oxidative and electrophile stress

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: osmC/ohr family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34762

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ohrA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by organic peroxides (OhrR) PubMed

• Regulatory mechanism:
  • OhrR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Minsun Hong, Mayuree Fuangthong, John D Helmann, Richard G Brennan
Structure of an OhrR-ohrA operator complex reveals the DNA binding mechanism of the MarR family.
Mol Cell: 2005, 20(1);131-41
[PubMed:16209951] [WorldCat.org] [DOI] (P p)

John D Helmann, Ming Fang Winston Wu, Ahmed Gaballa, Phil A Kobel, Maud M Morshedi, Paul Fawcett, Chris Paddon
The global transcriptional response of Bacillus subtilis to peroxide stress is coordinated by three transcription factors.
J Bacteriol: 2003, 185(1);243-53
[PubMed:12486061] [WorldCat.org] [DOI] (P p)

M Fuangthong, S Atichartpongkul, S Mongkolsuk, J D Helmann
OhrR is a repressor of ohrA, a key organic hydroperoxide resistance determinant in Bacillus subtilis.
J Bacteriol: 2001, 183(14);4134-41
[PubMed:11418552] [WorldCat.org] [DOI] (P p)

U Völker, K K Andersen, H Antelmann, K M Devine, M Hecker
One of two osmC homologs in Bacillus subtilis is part of the sigmaB-dependent general stress regulon.
J Bacteriol: 1998, 180(16);4212-8
[PubMed:9696771] [WorldCat.org] [DOI] (P p)