GcvT

• Description: aminomethyltransferase (glycine cleavage system protein T)
  
  

Gene name	gcvT
Synonyms	yqhI
Essential	no
Product	aminomethyltransferase (glycine cleavage system protein T)
Function	glycine utilization
MW, pI	39 kDa, 5.734
Gene length, protein length	1086 bp, 362 aa
Immediate neighbours	gcvPA, yqhH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU24570

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

utilization of amino acids

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: [Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH₃ (according to Swiss-Prot)

• Protein family: gcvT family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1YX2

• UniProt: P54378

• KEGG entry: [3]

• E.C. number: 2.1.2.10

Expression and regulation

• Operon: gcvT-gcvPA-gcvPB PubMed

• Sigma factor:

• Regulation:
  • induced by glycine PubMed

• Regulatory mechanism:
  • induction requires a co-operatively acting riboswitch (G-box) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion: pGP431 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Maumita Mandal, Mark Lee, Jeffrey E Barrick, Zasha Weinberg, Gail Mitchell Emilsson, Walter L Ruzzo, Ronald R Breaker
A glycine-dependent riboswitch that uses cooperative binding to control gene expression.
Science: 2004, 306(5694);275-9
[PubMed:15472076] [WorldCat.org] [DOI] (I p)

Jeffrey E Barrick, Keith A Corbino, Wade C Winkler, Ali Nahvi, Maumita Mandal, Jennifer Collins, Mark Lee, Adam Roth, Narasimhan Sudarsan, Inbal Jona, J Kenneth Wickiser, Ronald R Breaker
New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control.
Proc Natl Acad Sci U S A: 2004, 101(17);6421-6
[PubMed:15096624] [WorldCat.org] [DOI] (P p)