DesK

• Description: two-component sensor kinase, regulation of cold shock expression of des
  
  

Gene name	desK
Synonyms	yocF
Essential	no
Product	two-component sensor kinase
Function	regulation of cold shock expression of des
Metabolic function and regulation of this protein in SubtiPathways: Fatty acid degradation
MW, pI	42 kDa, 9.428
Gene length, protein length	1110 bp, 370 aa
Immediate neighbours	des, desR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU19190

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

lipid metabolism/ other, protein modification, transcription factors and their control, cold stress proteins, membrane proteins, phosphoproteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of DesR
• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • 5 transmembrane helices
  • cytoplasmatic C-terminal trail

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity: unsaturated fatty acids are negative effectors of the system

• Interactions: DesK-DesR

• Localization: membrane (transmembrane segments)

Database entries

• Structure: 3EHF

• UniProt: O34757

• KEGG entry: [3]

• E.C. number:

Additional information

• DesK has the ability to sense changes in membrane fluidity PubMed

Expression and regulation

• Operon: desK-desR PubMed

• Sigma factor:

• Regulation:
  • induced by cold shock (12-fold) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Diego de Mendoza, Universidad Nacional de Rosario, Argentine homepage
• Mohamed Marahiel, Marburg University, Germany homepage

References

Reviews

Richard C Stewart
Protein histidine kinases: assembly of active sites and their regulation in signaling pathways.
Curr Opin Microbiol: 2010, 13(2);133-41
[PubMed:20117042] [WorldCat.org] [DOI] (I p)

Pablo S Aguilar, Diego de Mendoza
Control of fatty acid desaturation: a mechanism conserved from bacteria to humans.
Mol Microbiol: 2006, 62(6);1507-14
[PubMed:17087771] [WorldCat.org] [DOI] (P p)