DacB

• Description: sporulation-specific penicillin-binding protein 5, D-alanyl-D-alanine carboxypeptidase
  
  

Gene name	dacB
Synonyms
Essential	no
Product	penicillin-binding protein 5, D-alanyl-D-alanine carboxypeptidase
Function	carboxypeptidase
MW, pI	42 kDa, 9.725
Gene length, protein length	1146 bp, 382 aa
Immediate neighbours	spmA, ypuI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU23190

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

cell wall synthesis, sporulation proteins, membrane proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala (according to Swiss-Prot)

• Protein family: peptidase S11 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure: 3MFD (beta-lactamase superfamily domain)

• UniProt: P35150

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dacB-spmA-spmB PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: