AsnH

• Description: asparagine synthase (glutamine-hydrolysing)
  
  

Gene name	asnH
Synonyms	yxaN
Essential	no
Product	asparagine synthase (glutamine-hydrolysing))
Function	biosynthesis of asparagine
Metabolic function and regulation of this protein in SubtiPathways: Asp, Asn
MW, pI	85 kDa, 5.528
Gene length, protein length	2241 bp, 747 aa
Immediate neighbours	yxnB, yxaM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU39920

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-aspartate + L-glutamine + H₂O = AMP + diphosphate + L-asparagine + L-glutamate (according to Swiss-Prot)

• Protein family: asparagine synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P42113

• KEGG entry: [3]

• E.C. number: 6.3.5.4

Expression and regulation

• Operon: yxbB-yxbA-yxnB-asnH-yxaM PubMed

• Sigma factor: sigA PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: