ArgJ

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  • Description: N-acetylglutamate synthase

Gene name argJ
Synonyms argA, argE
Essential no
Product N-acetylglutamate synthase
Function biosynthesis of arginine
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 43 kDa, 4.703
Gene length, protein length 1218 bp, 406 aa
Immediate neighbours argC, argB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ArgJ context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU11200

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate (according to Swiss-Prot)
  • Protein family: argJ family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

J Hlavácek, J Pícha, V Vanek, J Jirácek, J Slaninová, V Fucík, M Budĕsínský, D Gilner, R C Holz
Inhibitors of N(alpha)-acetyl-L-ornithine deacetylase: synthesis, characterization and analysis of their inhibitory potency.
Amino Acids: 2010, 38(4);1155-64
[PubMed:19649769] [WorldCat.org] [DOI] (I p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

A Mountain, N H Mann, R N Munton, S Baumberg
Cloning of a Bacillus subtilis restriction fragment complementing auxotrophic mutants of eight Escherichia coli genes of arginine biosynthesis.
Mol Gen Genet: 1984, 197(1);82-9
[PubMed:6096675] [WorldCat.org] [DOI] (P p)