LeuA

• Description: 2-isopropylmalate synthase
  
  

Gene name	leuA
Synonyms
Essential	no
Product	2-isopropylmalate synthase
Function	biosynthesis of leucine
Metabolic function and regulation of this protein in SubtiPathways: Ile, Leu, Val, Coenzyme A
MW, pI	56 kDa, 5.657
Gene length, protein length	1554 bp, 518 aa
Immediate neighbours	leuB, ilvC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU28280

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Acetyl-CoA + 3-methyl-2-oxobutanoate + H₂O = (2S)-2-isopropylmalate + CoA (according to Swiss-Prot)

• Protein family: LeuA type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot), membrane PubMed

Database entries

• Structure: 3EEG (from Cytophaga hutchinsonii atcc 33406, 53% identity, 68% similarity)

• UniProt: P94565

• KEGG entry: [3]

• E.C. number: 2.3.3.13

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: ilvB-ilvH-ilvC-leuA-leuB-leuC-leuD PubMed

• Sigma factor: SigA PubMed

• Regulation: for a complete overview on the regulation of the ilv operon, see Brinsmade et al.
  • repressed by casamino acids PubMed
  • expression is stimulated in the presence of glucose PubMed
  • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY, T-box) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • CcpA: transcription activation PubMed
  • T-box: tRNA-controlled RNA switch that mediates termination/antitermination
  • TnrA: transcription repression PubMed

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: