SigA

• Description: RNA polymerase major sigma factor SigA
  
  

Gene name	sigA
Synonyms	rpoD, crsA
Essential	yes PubMed
Product	RNA polymerase major sigma factor SigA
Function	transcription
MW, pI	42 kDa, 4.634
Gene length, protein length	1113 bp, 371 aa
Immediate neighbours	cccA, antE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU25200

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• List of SigA controlled genes

• Protein family: sigma-70 factor family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: Spo0A-SigA PubMed, SigA-(RpoB-RpoC) PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P06224

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • yqxD-dnaG-sigA PubMed
  • sigA PubMed

• Sigma factor:
  • yqxD: SigA PubMed, SigBPubMed, SigH PubMed
  • sigA: SigD PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Charles Moran, Emory University, NC, USA homepage

References

Amrita Kumar, James A Brannigan, Charles P Moran
Alpha-helix E of Spo0A is required for sigmaA- but not for sigmaH-dependent promoter activation in Bacillus subtilis.
J Bacteriol: 2004, 186(4);1078-83
[PubMed:14762002] [WorldCat.org] [DOI] (P p)

Claudia Rollenhagen, Haike Antelmann, Janine Kirstein, Olivier Delumeau, Michael Hecker, Michael D Yudkin
Binding of sigma(A) and sigma(B) to core RNA polymerase after environmental stress in Bacillus subtilis.
J Bacteriol: 2003, 185(1);35-40
[PubMed:12486038] [WorldCat.org] [DOI] (P p)

J Qiu, J D Helmann
Adenines at -11, -9 and -8 play a key role in the binding of Bacillus subtilis Esigma(A) RNA polymerase to -10 region single-stranded DNA.
Nucleic Acids Res: 1999, 27(23);4541-6
[PubMed:10556308] [WorldCat.org] [DOI] (P p)

G Schyns, C M Buckner, C P Moran
Activation of the Bacillus subtilis spoIIG promoter requires interaction of Spo0A and the sigma subunit of RNA polymerase.
J Bacteriol: 1997, 179(17);5605-8
[PubMed:9287022] [WorldCat.org] [DOI] (P p)

X Huang, F J Lopez de Saro, J D Helmann
sigma factor mutations affecting the sequence-selective interaction of RNA polymerase with -10 region single-stranded DNA.
Nucleic Acids Res: 1997, 25(13);2603-9
[PubMed:9185571] [WorldCat.org] [DOI] (P p)

J D Helmann
Compilation and analysis of Bacillus subtilis sigma A-dependent promoter sequences: evidence for extended contact between RNA polymerase and upstream promoter DNA.
Nucleic Acids Res: 1995, 23(13);2351-60
[PubMed:7630711] [WorldCat.org] [DOI] (P p)

S E Aiyar, Y L Juang, J D Helmann, P L deHaseth
Mutations in sigma factor that affect the temperature dependence of transcription from a promoter, but not from a mismatch bubble in double-stranded DNA.
Biochemistry: 1994, 33(38);11501-6
[PubMed:7918363] [WorldCat.org] [DOI] (P p)

J C Rong, J D Helmann
Genetic and physiological studies of Bacillus subtilis sigma A mutants defective in promoter melting.
J Bacteriol: 1994, 176(17);5218-24
[PubMed:8071196] [WorldCat.org] [DOI] (P p)

Y L Juang, J D Helmann
A promoter melting region in the primary sigma factor of Bacillus subtilis. Identification of functionally important aromatic amino acids.
J Mol Biol: 1994, 235(5);1470-88
[PubMed:8107087] [WorldCat.org] [DOI] (P p)

Additional publication: PubMed