InfB
- Description: translation initiation factor IF-2
Gene name | infB |
Synonyms | |
Essential | yes PubMed |
Product | initiation factor IF-2 |
Function | translation |
MW, pI | 78 kDa, 5.191 |
Gene length, protein length | 2148 bp, 716 aa |
Immediate neighbours | ylxQ, ylxP |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU16630
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: IF-2 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains: Nucleotide-binding domain that competitively binds GTP or ppGpp PubMed
- Modification:
- Cofactor(s):
- Effectors of protein activity: GTP or ppGpp PubMed
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure: 1D1N (fMet-tRNA-fMet binding domain, Geobacillus stearothermophilus), 1Z9B (C1-subdomain, Geobacillus stearothermophilus)
- UniProt: P17889
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information: Putative cellular metabolic sensor and regulator that oscillates between an active GTP-bound form under conditions allowing active protein syntheses and an inactive ppGpp-bound form when depleting of nutrients PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Pohl Milon, Eugene Tischenko, Jerneja Tomsic, Enrico Caserta, Gert Folkers, Anna La Teana, Marina V Rodnina, Cynthia L Pon, Rolf Boelens, Claudio O Gualerzi
The nucleotide-binding site of bacterial translation initiation factor 2 (IF2) as a metabolic sensor.
Proc Natl Acad Sci U S A: 2006, 103(38);13962-7
[PubMed:16968770]
[WorldCat.org]
[DOI]
(P p)
Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165]
[WorldCat.org]
[DOI]
(P p)
K Shazand, J Tucker, M Grunberg-Manago, J C Rabinowitz, T Leighton
Similar organization of the nusA-infB operon in Bacillus subtilis and Escherichia coli.
J Bacteriol: 1993, 175(10);2880-7
[PubMed:8491709]
[WorldCat.org]
[DOI]
(P p)