Methods

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Here you can find a list of methods for working with Bacillus subtilis:

  • Bacterial Two Hybrid: BACTH
  • SPINE: A method to detect in vivo protein-protein interactions
  • introduction of markerfree deletions: PubMed
  • generation of markerfree mutations: PubMed
  • Mechanical cell disruption and extraction of RNA from B. subtilis: Media:SOP-RNA.pdf
  • transformation of wild-type B. subtilis strains incl. NCIB3610: PubMed
  • Standard operation procedures (SOPs) of the SYSMO-BACELL consortium
  • in vivo detection of protein-protein interaction using DivIVA and GFP PubMed