CotY

• Description: spore coat protein (insoluble fraction), necessary for the assembly of the spre crust, the outermost layer of the spore coat
  
  

Gene name	cotY
Synonyms
Essential	no
Product	spore coat protein (insoluble fraction)
Function	spore coat assembly
MW, pI	17 kDa, 4.857
Gene length, protein length	486 bp, 162 aa
Immediate neighbours	cotZ, cotX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU11750

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: homotypic interaction PubMed, CotY-CotZ PubMed

• Localization: spore wall (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: Q08311

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cotY-cotZ PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK) PubMed

• Regulatory mechanism:
  • GerE: transcription activation PubMed
  • GerR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Peter T McKenney, Adam Driks, Haig A Eskandarian, Paul Grabowski, Jonathan Guberman, Katherine H Wang, Zemer Gitai, Patrick Eichenberger
A distance-weighted interaction map reveals a previously uncharacterized layer of the Bacillus subtilis spore coat.
Curr Biol: 2010, 20(10);934-8
[PubMed:20451384] [WorldCat.org] [DOI] (I p)

Daniela Krajcíková, Magda Lukácová, Denisa Müllerová, Simon M Cutting, Imrich Barák
Searching for protein-protein interactions within the Bacillus subtilis spore coat.
J Bacteriol: 2009, 191(10);3212-9
[PubMed:19304857] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Ritsuko Kuwana, Takashi Okumura, Hiromu Takamatsu, Kazuhito Watabe
The ylbO gene product of Bacillus subtilis is involved in the coat development and lysozyme resistance of spore.
FEMS Microbiol Lett: 2005, 242(1);51-7
[PubMed:15621419] [WorldCat.org] [DOI] (P p)

J Zhang, H Ichikawa, R Halberg, L Kroos, A I Aronson
Regulation of the transcription of a cluster of Bacillus subtilis spore coat genes.
J Mol Biol: 1994, 240(5);405-15
[PubMed:7519271] [WorldCat.org] [DOI] (P p)