GutB

• Description: D-sorbitol dehydrogenase
  
  

Gene name	gutB
Synonyms
Essential	no
Product	D-sorbitol dehydrogenase
Function	glucitol utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	38 kDa, 5.213
Gene length, protein length	1059 bp, 353 aa
Immediate neighbours	gutR, gutP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU06150

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-iditol + NAD⁺ = L-sorbose + NADH (according to Swiss-Prot)

• Protein family: zinc-containing alcohol dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: Q06004

• KEGG entry: [3]

• E.C. number: 1.1.1.14

Expression and regulation

• Operon: gutB-gutP PubMed

• Sigma factor: SigA PubMed

• Regulation: induced by glucitol (GutR) PubMed

• Regulatory mechanism: GutR: transcriptional activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Shouji Watanabe, Miyuki Hamano, Hiroshi Kakeshita, Keigo Bunai, Shigeo Tojo, Hirotake Yamaguchi, Yasutaro Fujita, Sui-Lam Wong, Kunio Yamane
Mannitol-1-phosphate dehydrogenase (MtlD) is required for mannitol and glucitol assimilation in Bacillus subtilis: possible cooperation of mtl and gut operons.
J Bacteriol: 2003, 185(16);4816-24
[PubMed:12897001] [WorldCat.org] [DOI] (P p)

R Ye, S L Wong
Transcriptional regulation of the Bacillus subtilis glucitol dehydrogenase gene.
J Bacteriol: 1994, 176(11);3314-20
[PubMed:8195086] [WorldCat.org] [DOI] (P p)

K Ng, R Ye, X C Wu, S L Wong
Sorbitol dehydrogenase from Bacillus subtilis. Purification, characterization, and gene cloning.
J Biol Chem: 1992, 267(35);24989-94
[PubMed:1460002] [WorldCat.org] (P p)