List of plasmids

Here you can find a list of Bacillus subtilis plasmids:

Fusion to reporter genes

• pAC5: translational lacZ-fusions (chloramphenicol resistence)
• pAC6: transcriptional lacZ-fusions (cat resistence)
• pAC7: translational lacZ-fusions (kanamycin resistence)
• pBaSysBioII: gfp transcriptional fusions for high-throughput analysis of gene expression PubMed

Labelling of a protein with a triple FLAG tag

• pGP1331: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter
• pGP1087: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter, allowing the expression of downstream genes under the control of the IPTG-inducible Pspac promoter

Expression/ purification in B. subtilis

• pBQ200: overexpression of proteins in B. subtilis under control of a strong degQ36 promoter
• pGP380: expression of proteins in B. subtilis allows fusion to a Strep-tag at the N-terminus of the protein
• pGP382: expression of proteins in B. subtilis allows fusion to a Strep-tag at the C-terminus of the protein

Expression/ purification in E. coli

• pWH844: expression of proteins in E. coli, allows fusion to a N-terminal His-tag
• pGP570: expression of proteins in E. coli, allows fusion to a N-terminal His-tag that can be cleaved off
• pGP172: expression of proteins in E. coli allows fusion to a Strep-tag at the N-terminus of the protein
• pGP574: expression of proteins in E. coli allows fusion to a Strep-tag at the C-terminus of the protein
• pGP1755: expression of a mutated malatedehydrogenase from B. subtilis in E. coli"; mdh (Ser-149-Ala) Bam+Pst in pWH844