GalE

• Description: UDP glucose 4-epimerase
  
  

Gene name	galE
Synonyms
Essential	no
Product	UDP glucose 4-epimerase
Function	galactose utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	36 kDa, 4.776
Gene length, protein length	1017 bp, 339 aa
Immediate neighbours	yxkC, yxkA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU38860

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: UDP-glucose = UDP-galactose (according to Swiss-Prot)

• Protein family: sugar epimerase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1LRL (from Escherichia coli Y299C mutant, 57% identity, 71% similarity) PubMed

• UniProt: P55180

• KEGG entry: [3]

• E.C. number: 5.1.3.2

Expression and regulation

• Operon: galE PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Blazenka Soldo, Claudio Scotti, Dimitri Karamata, Vladimir Lazarevic
The Bacillus subtilis Gne (GneA, GalE) protein can catalyse UDP-glucose as well as UDP-N-acetylglucosamine 4-epimerisation.
Gene: 2003, 319;65-9
[PubMed:14597172] [WorldCat.org] [DOI] (P p)

O Krispin, R Allmansberger
The Bacillus subtilis galE gene is essential in the presence of glucose and galactose.
J Bacteriol: 1998, 180(8);2265-70
[PubMed:9555917] [WorldCat.org] [DOI] (P p)