Sda

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  • Description: developmental checkpoint protein, inhibits the autokinase activity of KinA and KinB, control of the phosphorelay


Gene name sda
Synonyms
Essential no
Product developmental checkpoint protein
Function mediates a developmental checkpoint coupling
initiation of sporulation (phosphorylation of Spo0A)
to the function of replication initiation proteins
Function and regulation of this protein in SubtiPathways:
Phosphorelay
MW, pI 6 kDa, 5.605
Gene length, protein length 156 bp, 52 aa
Immediate neighbours yloV, yqeF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Sda context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU25690

Phenotypes of a mutant

  • sporulates at a higher frequency
  • will sporulate in the presence of replication stress

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Sda is a checkpoint protein that is upregulated in response to replication stress PubMed. Sda inhibits the autokinase activity of KinA (and likely KinB as well). This in its turn results in reduced levels of Spo0A~P. Thus, replication stressed cells will not initiate sporulation.
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure: 3D36 (from Geobacillus stearothermophilus, complex with KinB), 1PV0
  • KEGG entry: [3]
  • E.C. number:

Additional information

Sda is rapidly turned over by the ClpP/X system because of some uncharged residues at its C-terminus PubMed.

Expression and regulation

  • Regulatory mechanism:
  • Additional information: Sda is rapidly turned over by the ClpP/X system because of some uncharged residues at its C-terminus PubMed.

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Bill Burkholder Homepage

Your additional remarks

References