ThiE

• Description: thiamine-phosphate diphosphorylase
  
  

Gene name	thiE
Synonyms	thiC, ipa-26d, ywbK
Essential	no
Product	thiamine-phosphate diphosphorylase
Function	biosynthesis of thiamine by 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate to yield thiamine phosphate
Metabolic function and regulation of this protein in SubtiPathways: Thiamin
MW, pI	23 kDa, 5.058
Gene length, protein length	666 bp, 222 aa
Immediate neighbours	ywbL, thiM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU38290

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate (according to Swiss-Prot)

• Protein family: TMP-PPase family (according to Swiss-Prot)

• Paralogous protein(s): TenI

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1G6C

• UniProt: P39594

• KEGG entry: [3]

• E.C. number: 2.5.1.3

Expression and regulation

• Operon: ywbI-thiM-thiE PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

Original publications

Y Zhang, S V Taylor, H J Chiu, T P Begley
Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis.
J Bacteriol: 1997, 179(9);3030-5
[PubMed:9139923] [WorldCat.org] [DOI] (P p)