TcyM

• Description: cystine ABC transporter (permease)
  
  

Gene name	tcyM
Synonyms	ytmM
Essential	no
Product	cystine ABC transporter (permease)
Function	cystine uptake
Metabolic function and regulation of this protein in SubtiPathways: Riboflavin and FAD synthesis
MW, pI	25 kDa, 8.767
Gene length, protein length	705 bp, 235 aa
Immediate neighbours	tcyN, tcyL
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU29350

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: binding-protein-dependent transport system permease family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O34931

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ytmI-tcyJ-tcyK-tcyL-tcyM-tcyN-ytmO-ytnI-ytnJ-ribR-ytnL-ytnM PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of cysteine (CymR) PubMed

• Regulatory mechanism:
  • CymR: transcription repression PubMed
  • YtlI: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Pierre Burguière, Juliette Fert, Isabelle Guillouard, Sandrine Auger, Antoine Danchin, Isabelle Martin-Verstraete
Regulation of the Bacillus subtilis ytmI operon, involved in sulfur metabolism.
J Bacteriol: 2005, 187(17);6019-30
[PubMed:16109943] [WorldCat.org] [DOI] (P p)

Irina M Solovieva, Rimma A Kreneva, Lubov Errais Lopes, Daniel A Perumov
The riboflavin kinase encoding gene ribR of Bacillus subtilis is a part of a 10 kb operon, which is negatively regulated by the yrzC gene product.
FEMS Microbiol Lett: 2005, 243(1);51-8
[PubMed:15668000] [WorldCat.org] [DOI] (P p)

I M Solov'eva, R A Kreneva, L L Erraĭs, A S Mironov, D A Perumov
[Study of the mechanism for regulating ribR gene activity in Bacillus subtilis]. [Issledovanie mekhanizma reguliatsii aktivnosti gena ribR u Bacillus subtilis.]
Genetika: 2004, 40(5);716-20
[PubMed:15272571] [WorldCat.org] (P p)

Pierre Burguière, Sandrine Auger, Marie-Françoise Hullo, Antoine Danchin, Isabelle Martin-Verstraete
Three different systems participate in L-cystine uptake in Bacillus subtilis.
J Bacteriol: 2004, 186(15);4875-84
[PubMed:15262924] [WorldCat.org] [DOI] (P p)

Sandrine Auger, Antoine Danchin, Isabelle Martin-Verstraete
Global expression profile of Bacillus subtilis grown in the presence of sulfate or methionine.
J Bacteriol: 2002, 184(18);5179-86
[PubMed:12193636] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)