GlyA

• Description: serine hydroxymethyltransferase
  
  

Gene name	glyA
Synonyms	glyC, ipc-34d
Essential	yes PubMed
Product	serine hydroxymethyltransferase
Function	biosynthesis of glycine
Metabolic function and regulation of this protein in SubtiPathways: Ala, Gly, Ser
MW, pI	45 kDa, 5.475
Gene length, protein length	1245 bp, 415 aa
Immediate neighbours	upp, ywlG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU36900

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5,10-methylenetetrahydrofolate + glycine + H₂O = tetrahydrofolate + L-serine (according to Swiss-Prot)

• Protein family: SHMT family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on ser/ thr/ tyr PubMed, PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2VGU (complex with L-serine, Geobacillus stearothermophilus), 2VI8 (Geobacillus stearothermophilus)

• UniProt: P39148

• KEGG entry: [3]

• E.C. number: 2.1.2.1

Expression and regulation

• Operon: glyA (according to http://dbtbs.hgc.jp/COG/prom/glyA.html DBTBS)

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of adenine or adenosine(PurR) PubMed
  • induced by glycine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed
  • PurR: transcription repression (molecular inducer: PRPP) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: