IseA

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  • Description: inhibits in vitro activity of cell wall endopeptidases LytE and LytF, inhibits cell separation

Gene name iseA
Synonyms yoeB
Essential no
Product inhibitor of autolysins
Function protection against cell envelope stress
MW, pI 19 kDa, 10.176
Gene length, protein length 543 bp, 181 aa
Immediate neighbours yoeA, trnSL-Arg1
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YoeB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU18380

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions: IseA-LytF (catalytic domain)
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulatory mechanism:
  • WalR: transcription repression PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Hiroki Yamamoto, Masayuki Hashimoto, Yuhei Higashitsuji, Hiroyuki Harada, Nozomi Hariyama, Lisa Takahashi, Tomoaki Iwashita, Seika Ooiwa, Junichi Sekiguchi
Post-translational control of vegetative cell separation enzymes through a direct interaction with specific inhibitor IseA in Bacillus subtilis.
Mol Microbiol: 2008, 70(1);168-82
[PubMed:18761694] [WorldCat.org] [DOI] (I p)

Paola Bisicchia, David Noone, Efthimia Lioliou, Alistair Howell, Sarah Quigley, Thomas Jensen, Hanne Jarmer, Kevin M Devine
The essential YycFG two-component system controls cell wall metabolism in Bacillus subtilis.
Mol Microbiol: 2007, 65(1);180-200
[PubMed:17581128] [WorldCat.org] [DOI] (P p)

Letal I Salzberg, John D Helmann
An antibiotic-inducible cell wall-associated protein that protects Bacillus subtilis from autolysis.
J Bacteriol: 2007, 189(13);4671-80
[PubMed:17483219] [WorldCat.org] [DOI] (P p)