LevB

• Description: endolevanase
  
  

Gene name	levB
Synonyms	yveB
Essential	no
Product	endolevanase
Function	levan degradation
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	58 kDa, 4.993
Gene length, protein length	1548 bp, 516 aa
Immediate neighbours	sacB, yveA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU34460

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of (2->6)-beta-D-fructofuranan, to remove successive disaccharide residues as levanbiose, i (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 32 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O07003

• KEGG entry: [3]

• E.C. number: 3.2.1.64

Expression and regulation

• Operon: sacB-levB-yveA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induction by sucrose (at high concentration) (SacY) PubMed

• Regulatory mechanism:
  • SacY: binding to a RNA switch (transcriptional antitermination) PubMed
  • DegU-P: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: