BglS

• Description: endo-beta-1,3-1,4 glucanase
  
  

Gene name	bglS
Synonyms	bgl, licS
Essential	no
Product	endo-beta-1,3-1,4 glucanase
Function	lichenan degradation
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	27 kDa, 6.482
Gene length, protein length	726 bp, 242 aa
Immediate neighbours	citH, licT
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU39070

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 16 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide) PubMed

Database entries

• Structure:

• UniProt: P04957

• KEGG entry: [3]

• E.C. number: 3.2.1.73 3

Expression and regulation

• Operon:
  • licT-bglS PubMed
  • bglS PubMed

• Sigma factor:
  • licT: SigA PubMed

• Regulation:
  • repressed by glucose (3.1-fold) (CcpA) PubMed
  • expressed in the stationary phase (temporal activation) PubMed
  • weak induction by ß-glucosides (LicT) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • LicT: binding to an RNA switch results in transcriptional antitermination PubMed

• Additional information:

Biological materials

• Mutant: GP427 (licT-bglS, erm), BGW7 (cat), both available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: