PGP382

pGP382: click to enlarge

pGP382 cloning region: click to enlarge

Vector for the overexpression of C-terminally Strep-tagged proteins in B. subtilis, used for the SPINE technology

constructed in Stülke lab

in E. coli: ampicillin resistance

in B. subtilis: erythromycin resistance

based on pBQ200

similar vector: pGP380

sequencing primer:

• M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
• M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘

Christina Herzberg, Lope Andrés Flórez Weidinger, Bastian Dörrbecker, Sebastian Hübner, Jörg Stülke, Fabian M Commichau
SPINE: a method for the rapid detection and analysis of protein-protein interactions in vivo.
Proteomics: 2007, 7(22);4032-5
[PubMed:17994626] [WorldCat.org] [DOI] (P p)