TagD

• Description: glycerol-3-phosphate cytidylyltransferase
  
  

Gene name	tagD
Synonyms
Essential	yes PubMed
Product	glycerol-3-phosphate cytidylyltransferase
Function	biosynthesis of teichoic acid
MW, pI	15 kDa, 5.672
Gene length, protein length	387 bp, 129 aa
Immediate neighbours	tagE, tagA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU35740

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol (according to Swiss-Prot)

• Protein family: cytidylyltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: TagD-TagD

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1N1D (complex with CDP-glycerol), 1COZ

• UniProt: P27623

• KEGG entry: [3]

• E.C. number: 2.7.7.39

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • repressed when phosphate is available (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

A P Bhavsar, T J Beveridge, E D Brown
Precise deletion of tagD and controlled depletion of its product, glycerol 3-phosphate cytidylyltransferase, leads to irregular morphology and lysis of Bacillus subtilis grown at physiological temperature.
J Bacteriol: 2001, 183(22);6688-93
[PubMed:11673441] [WorldCat.org] [DOI] (P p)

C H Weber, Y S Park, S Sanker, C Kent, M L Ludwig
A prototypical cytidylyltransferase: CTP:glycerol-3-phosphate cytidylyltransferase from bacillus subtilis.
Structure: 1999, 7(9);1113-24
[PubMed:10508782] [WorldCat.org] [DOI] (P p)

Y Qi, F M Hulett
Role of Pho-P in transcriptional regulation of genes involved in cell wall anionic polymer biosynthesis in Bacillus subtilis.
J Bacteriol: 1998, 180(15);4007-10
[PubMed:9683503] [WorldCat.org] [DOI] (P p)

W Liu, S Eder, F M Hulett
Analysis of Bacillus subtilis tagAB and tagDEF expression during phosphate starvation identifies a repressor role for PhoP-P.
J Bacteriol: 1998, 180(3);753-8
[PubMed:9457886] [WorldCat.org] [DOI] (P p)