AmyX

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  • Description: pullulanase (debranching enzyme)

Gene name amyX
Synonyms
Essential no
Product pullulanase
Function starch degradation
MW, pI 80 kDa, 5.799
Gene length, protein length 2154 bp, 718 aa
Immediate neighbours ytmP, ytlR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AmyX context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU29930

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): GlgB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 2E8Z (complex with alpha cyclodextrin), 2E9B (complex with maltose)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jae-Hoon Shim, Jong-Tae Park, Jung-Sun Hong, Ki Woo Kim, Myo-Jeong Kim, Jung-Hyuk Auh, Young-Wan Kim, Cheon-Seok Park, Winfried Boos, Jung-Wan Kim, Kwan-Hwa Park
Role of maltogenic amylase and pullulanase in maltodextrin and glycogen metabolism of Bacillus subtilis 168.
J Bacteriol: 2009, 191(15);4835-44
[PubMed:19465663] [WorldCat.org] [DOI] (I p)

Dominggus Malle, Takafumi Itoh, Wataru Hashimoto, Kousaku Murata, Shigeru Utsumi, Bunzo Mikami
Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2006, 62(Pt 4);381-4
[PubMed:16582490] [WorldCat.org] [DOI] (I p)