LcfB

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  • Description: long-chain fatty-acid-CoA ligase

Gene name lcfB
Synonyms yhfL
Essential no
Product long-chain fatty-acid-CoA ligase
Function fatty acid degradation
MW, pI 56 kDa, 5.301
Gene length, protein length 1539 bp, 513 aa
Immediate neighbours yhfK, yhfM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YhfL context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU10270

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot)
  • Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • repressed in the absence of long-chain fatty acids (FadR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Matsuoka H, Hirooka K, Fujita Y. (2007) Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation. J Biol Chem. Feb 23;282(8): 5180-94. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed