NasE

From SubtiWiki
Revision as of 12:34, 12 June 2009 by Sroppel (talk | contribs)
Jump to: navigation, search
  • Description: assimilatory nitrite reductase (subunit)

Gene name nasE
Synonyms nasBD, nirD
Essential no
Product assimilatory nitrite reductase (subunit)
Function utilization of nitrite as nitrogen source
MW, pI 11 kDa, 4.805
Gene length, protein length 318 bp, 106 aa
Immediate neighbours nasF, nasD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NasE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU03290

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Ammonium hydroxide + 3 NAD(P)+ + H2O = nitrite + 3 NAD(P)H (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • expressed under anaerobic conditions (ResD) PubMed
    • repressed unless NO is present under anaerobic conditions (NsrR) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed