Sandbox

• Description: cystathionine b-lyase
  
  

Gene name	metC
Synonyms	yjcJ
Essential	no
Product	cystathionine b-lyase
Function	biosynthesis of methionine
MW, pI	42 kDa, 5.757
Gene length, protein length	1170 bp, 390 aa
Immediate neighbours	metI, yjcK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU11880

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-cystathionine + H₂O = L-homocysteine + NH₃ + pyruvate (according to Swiss-Prot)

• Protein family: trans-sulfuration enzymes family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: O31632

• KEGG entry: [3]

• E.C. number: 4.4.1.8

Expression and regulation

• Operon: metI-metC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • induced by methionine starvation (S-box) PubMed

• Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

1. Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed