Sandbox

• Description: glycine decarboxylase (subunit 2)
  
  

Gene name	gcvPB
Synonyms	yqhK, gcvP
Essential	no
Product	glycine decarboxylase (subunit 2)
Function	glycine utilization
MW, pI	54 kDa, 5.283
Gene length, protein length	1464 bp, 488 aa
Immediate neighbours	yqhL, gcvPA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU24550

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Glycine + H-protein-lipoyllysine = H-protein-S-aminomethyldihydrolipoyllysine + CO₂ (according to Swiss-Prot)

• Protein family: C-terminal subunit subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: membrane (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P54377

• KEGG entry: [3]

• E.C. number: 1.4.4.2

Expression and regulation

• Operon: gcvT-gcvPA-gcvPB PubMed

• Sigma factor:

• Regulation: repressed by casamino acids PubMed , induced by glycine PubMed

• Regulatory mechanism: induction requires a co-operatively acting riboswitch PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Maumita Mandal, Mark Lee, Jeffrey E Barrick, Zasha Weinberg, Gail Mitchell Emilsson, Walter L Ruzzo, Ronald R Breaker
A glycine-dependent riboswitch that uses cooperative binding to control gene expression.
Science: 2004, 306(5694);275-9
[PubMed:15472076] [WorldCat.org] [DOI] (I p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

1. Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
2. Mandal M, Lee M, Barrick JE, Weinberg Z, Emilsson GM, Ruzzo WL, Breaker RR (2004) A glycine-dependent riboswitch that uses cooperative binding to control gene expression. Science 306: 275-279. PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed