DegS

• Description: two-component sensor kinase for exoenzyme and competence regulation
  
  

Gene name	degS
Synonyms	sacU
Essential	no
Product	two-component sensor kinase (NarL family)
Function	regulation of degradative enzyme and genetic competence
MW, pI	44 kDa, 5.988
Gene length, protein length	1155 bp, 385 aa
Immediate neighbours	degU, yvyE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine (according to Swiss-Prot) autophosphorylation, autodephosphorylation, phosphorylation of DegU on Asp-56 PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• • aa 12 ... 170: unknown DegS domain
  • aa 180 ... 248: HisKA_3 domain (autophosphorylation)
  • aa 289 ... 384: HATPase_c

• Modification: phosphorylation on Ser-76 PubMed, autophosphorylation on His-189

• Cofactor(s):

• Effectors of protein activity: DegQ

• Interactions: DegS-DegU, DegS-DegQ

• Localization: Cytoplasm (Homogeneous) PubMed

Database entries

• Structure:

• Swiss prot entry: P13799

• KEGG entry: BSU35500

• E.C. number:

Expression and regulation

• Operon: degS-degU

• Sigma factor: SigA

• Regulation: induced in the presence of ammonium (TnrA)

• Regulatory mechanism: TnrA: repression, DegU: positive autoregulation

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Tarek Msadek, Institut Pasteur, Paris, France

References

1. Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory Proteomics 6: 2135-2146. PubMed
2. Macek et al. (2007) The serine/ threonine/ tyrosine phosphoproteome of the model bacterium Bacillus subtilis. Mol. Cell. Proteomics 6: 697-707 PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed