Rnc

• Description: RNase III
  
  

Gene name	rnc
Synonyms	rncS
Essential	yes
Product	endoribonuclease III
Function	cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
MW, pI	28 kDa, 8.076
Gene length, protein length	747 bp, 249 aa
Immediate neighbours	acpA, smc
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Phenotypes of a mutant

essential PubMed

rnc is essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry:

• KEGG entry: [3]

• E.C. number: 3.1.26.3

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

References

1. Wang, W. & Bechhofer, D. H. (1997). Bacillus subtilis RNase III gene: cloning, function of the gene in Escherichia coli, and construction of Bacillus subtilis strains with altered rnc loci. J Bacteriol 179, 7379-7385. PubMed
2. Herskovitz, M. A. & Bechhofer, D. H. (2000). Endoribonuclease RNase III is essential in Bacillus subtilis. Mol Microbiol 38:1027-1033. PubMed
3. Mitra, S. & Bechhofer, D. H. (1994). Substrate specificity of an RNase III-like activity from Bacillus subtilis. J Biol Chem 269:31450-31456. PubMed
4. Oguro, A., Kakeshita, H., Nakamura, K., Yamane, K., Wang, W. & Bechhofer, D. H. (1998). Bacillus subtilis RNase III cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor. J Biol Chem 273:19542-19547. PubMed
5. Yao, S., Blaustein, J. B. & Bechhofer, D. H. (2007). Processing of Bacillus subtilis small cytoplasmic RNA: evidence for an additional endonuclease cleavage site. Nucleic Acids Res. 35:4464-4473. PubMed
6. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed