SigD
- Description: RNA polymerase sigma factor SigD
Gene name | sigD |
Synonyms | flaB |
Essential | no |
Product | RNA polymerase sigma factor SigD |
Function | regulation of flagella, motility, chemotaxis and autolysis |
Gene expression levels in SubtiExpress: sigD | |
Interactions involving this protein in SubtInteract: SigD | |
MW, pI | 29 kDa, 5.167 |
Gene length, protein length | 762 bp, 254 aa |
Immediate neighbours | cheD, swrB |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
transcription, sigma factors and their control, motility and chemotaxis
This gene is a member of the following regulons
CodY regulon, DegU regulon, SigD regulon, Spo0A regulon
The SigD regulon
The gene
Basic information
- Locus tag: BSU16470
Phenotypes of a mutant
- Inactivation of alsR, sigD and sigW increases competitive fitness of Bacillus subtilis under non-sporulating conditions PubMed
- mucoid phenotype due to the overproduction of poly-gamma-glutamate (because of the loss of motA-motB expression) PubMed
- not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed
Database entries
- BsubCyc: BSU16470
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: sigma-70 factor family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
Database entries
- BsubCyc: BSU16470
- Structure:
- UniProt: P10726
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: part of the fla-che operon
- flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
- ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
- sigD-swrB PubMed
- Regulation: see fla-che operon
- Regulatory mechanism: see fla-che operon
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
The SigD regulon
Original publications