KinC

• Description: two-component sensor kinase, phosphorylates Spo0F and Spo0A, part of the phosphorelay, governs expression of genes involved in biofilm formation
  
  

Gene name	kinC
Synonyms	ssb
Essential	no
Product	two-component sensor kinase
Function	initiation of sporulation
Gene expression levels in SubtiExpress: kinC
Interactions involving this protein in SubtInteract: KinC
Function and regulation of this protein in SubtiPathways: kinC
MW, pI	48 kDa, 6.225
Gene length, protein length	1284 bp, 428 aa
Immediate neighbours	abh, ykqA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, transcription factors and their control, phosphorelay, biofilm formation, membrane proteins, phosphoproteins

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

• Locus tag: BSU14490

Phenotypes of a mutant

• • defective in biofilm formation PubMed

Database entries

• BsubCyc: BSU14490

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • autophosphorylation, phosphorylation of Spo0F as part of the phosphorelay, but also direct phosphorylation of Spo0A PubMed
  • mainly active in the younger, outer regions of a colony (with KinD) PubMed
  • phosphorylates Spo0A in response to the presence of surfactin PubMed, this has been refuted PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • two transmembrane segments
  • PAS domain
  • C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:
  • activity is triggered by potassium leakage PubMed, this has been refuted PubMed
  • activity is triggered by polyisoprenoid lipids formed by YisP PubMed

• Interactions:
  • KinC-Spo0A PubMed
  • KinC-Spo0F

• Localization:
  • cell membrane (Heterogeneous) PubMed
  • co-localizes with FloT in discrete foci in the membrane PubMed
  • the localization of KinC in membrane microdomains depends on FloA and FloT PubMed, this has been refuted PubMed

Database entries

• BsubCyc: BSU14490

• Structure:

• UniProt: P39764

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: kinC (according to DBTBS)

• Expression browser: kinC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A632 ( kinC::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Marc Bramkamp, Daniel Lopez
Exploring the existence of lipid rafts in bacteria.
Microbiol Mol Biol Rev: 2015, 79(1);81-100
[PubMed:25652542] [WorldCat.org] [DOI] (I p)