YcnK

• Description: copper-responsive transcription repressor of the ycnK-ycnJ-ycnI operon
  
  

Gene name	ycnK
Synonyms
Essential	no
Product	transcription repressor, DeoR family
Function	regulation of copper uptake
Gene expression levels in SubtiExpress: ycnK
MW, pI	21 kDa, 7.371
Gene length, protein length	570 bp, 190 aa
Immediate neighbours	ycnJ, ycnL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), transcription factors and their control

This gene is a member of the following regulons

AbrB regulon, YcnK regulon

The YcnK regulon:

• ycnK-ycnJ-ycnI

The gene

Basic information

• Locus tag: BSU03960

Phenotypes of a mutant

• elevated expression of ycnJ under conditions of copper excess PubMed

Database entries

• BsubCyc: BSU03960

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: repression of ycnK-ycnJ-ycnI expression in the presence of excess copper PubMed

• Protein family: DeoR family of transcription factors

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): copper acts as corepressor PubMed

• Effectors of protein activity:

• Interactions:
  • forms dimers PubMed

• Localization:

Database entries

• BsubCyc: BSU03960

• Structure:

• UniProt: P94433

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ycnK-ycnJ-ycnI PubMed

• Expression browser: ycnK PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during logrithmic growth (AbrB) PubMed
  • induced by copper limitation (YcnK) PubMed
  • induction of the ycnK-ycnJ-ycnI in a csoR mutant results from intracellular copper depletion due to the constitutive expression of the copZ-copA operon (copper export) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed
  • YcnK: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage

References

Reviews

Corinna Rademacher, Bernd Masepohl
Copper-responsive gene regulation in bacteria.
Microbiology (Reading): 2012, 158(Pt 10);2451-2464
[PubMed:22918892] [WorldCat.org] [DOI] (I p)