SpoIIIJ

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  • Description: Sec-independent membrane protein translocase, essential for SigG activity at stage III

Gene name spoIIIJ
Synonyms spo0J87
Essential no
Product membrane protein translocase
Function membrane insertion of proteins and protein secretion
Gene expression levels in SubtiExpress: spoIIIJ
Interactions involving this protein in SubtInteract: SpoIIIJ
MW, pI 29 kDa, 9.992
Gene length, protein length 783 bp, 261 aa
Immediate neighbours jag, rnpA
Sequences Protein DNA DNA_with_flanks
Genetic context
ThdF jag spoIIIJ rnpA rpmH context.png
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SpoIIIJ expression.png















Categories containing this gene/protein

protein secretion, sporulation/ other, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU41040

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: membrane protein translocase, facilitates insertion of SpoIIIAE into the membrane PubMed
  • Protein family: YidC/Oxa1/Alb3 family PubMed
  • Paralogous protein(s): YidC2, one of the two proteins has to be present for viability PubMed

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 1044 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Richard Losick, Harvard University, Cambridge, USA homepage

Adriano Henriques, Lisbon, Portugal homepage

Your additional remarks

References

Reviews

Ross E Dalbey, Peng Wang, Jan Maarten van Dijl
Membrane proteases in the bacterial protein secretion and quality control pathway.
Microbiol Mol Biol Rev: 2012, 76(2);311-30
[PubMed:22688815] [WorldCat.org] [DOI] (I p)

Soledad Funes, Frank Kauff, Eli O van der Sluis, Martin Ott, Johannes M Herrmann
Evolution of YidC/Oxa1/Alb3 insertases: three independent gene duplications followed by functional specialization in bacteria, mitochondria and chloroplasts.
Biol Chem: 2011, 392(1-2);13-9
[PubMed:21194367] [WorldCat.org] [DOI] (I p)

Peng Wang, Ross E Dalbey
Inserting membrane proteins: the YidC/Oxa1/Alb3 machinery in bacteria, mitochondria, and chloroplasts.
Biochim Biophys Acta: 2011, 1808(3);866-75
[PubMed:20800571] [WorldCat.org] [DOI] (P p)

Martin van der Laan, Nico P Nouwen, Arnold J M Driessen
YidC--an evolutionary conserved device for the assembly of energy-transducing membrane protein complexes.
Curr Opin Microbiol: 2005, 8(2);182-7
[PubMed:15802250] [WorldCat.org] [DOI] (P p)


Original publications