ArgS

• Description: arginyl-tRNA synthetase, universally conserved protein
  
  

Gene name	argS
Synonyms
Essential	yes PubMed
Product	arginyl-tRNA synthetase
Function	translation
Gene expression levels in SubtiExpress: argS
Metabolic function and regulation of this protein in SubtiPathways: argS
MW, pI	62 kDa, 5.11
Gene length, protein length	1668 bp, 556 aa
Immediate neighbours	narK, ywiB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, universally conserved proteins

The gene

Basic information

• Locus tag: BSU37330

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU37330

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg) (according to Swiss-Prot)

• Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU37330

• Structure: 3FNR (from Campylobacter jejuni, 39% identity, 56% similarity)

• UniProt: P46906

• KEGG entry: [3]

• E.C. number: 6.1.1.19

Additional information

universally conserved protein

Expression and regulation

• Operon: argS PubMed

• Expression browser: argS PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1206 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 3289 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1716 PubMed

• • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 3749 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

E Presecan, I Moszer, L Boursier, H Cruz Ramos, V de la Fuente, M-F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-3328
[PubMed:9353933] [WorldCat.org] [DOI] (P p)