Apt

• Description: adenine phosphoribosyltransferase, universally conserved protein
  
  

Gene name	apt
Synonyms
Essential	no
Product	adenine phosphoribosyltransferase
Function	purine salvage and interconversion
Gene expression levels in SubtiExpress: apt
Metabolic function and regulation of this protein in SubtiPathways: apt
MW, pI	18 kDa, 4.701
Gene length, protein length	510 bp, 170 aa
Immediate neighbours	relA, yrvE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, universally conserved proteins, phosphoproteins

The gene

Basic information

• Locus tag: BSU27610

Database entries

• BsubCyc: BSU27610

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)

• Protein family: purine/pyrimidine phosphoribosyltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-85 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU27610

• Structure: 2DY0 (from Escherichia coli k12, 56% identity, 66% similarity)

• UniProt: O34443

• KEGG entry: [3]

• E.C. number: 2.4.2.7

Additional information

universally conserved protein

Expression and regulation

• Operon: yrvE-apt PubMed

• Expression browser: apt PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 872 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2750 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1189 PubMed

• • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1706 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc Natl Acad Sci U S A: 2012, 109(19);7451-6
[PubMed:22517742] [WorldCat.org] [DOI] (I p)

M Nickel, G Homuth, C Böhnisch, U Mäder, T Schweder
Cold induction of the Bacillus subtilis bkd operon is mediated by increased mRNA stability.
Mol Genet Genomics: 2004, 272(1);98-107
[PubMed:15241682] [WorldCat.org] [DOI] (P p)

H H Saxild, P Nygaard
Genetic and physiological characterization of Bacillus subtilis mutants resistant to purine analogs.
J Bacteriol: 1987, 169(7);2977-83
[PubMed:3110131] [WorldCat.org] [DOI] (P p)