AcsA

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  • Description: acetyl-CoA synthetase

Gene name acsA
Synonyms
Essential no
Product acetyl-CoA synthetase)
Function utilization of acetate, fatty acids
Gene expression levels in SubtiExpress: acsA
Metabolic function and regulation of this protein in SubtiPathways:
acsA
MW, pI 64 kDa, 5.547
Gene length, protein length 1716 bp, 572 aa
Immediate neighbours ytzK, acuA
Sequences Protein DNA DNA_with_flanks
Genetic context
AcsA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AcsA expression.png




























Categories containing this gene/protein

utilization of specific carbon sources, utilization of lipids

This gene is a member of the following regulons

CcpA regulon, CodY regulon

The gene

Basic information

  • Locus tag: BSU29680

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA (according to Swiss-Prot)
  • Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: acetylated on Lys-549 by AcuA, this results in inactivation PubMed, deacetylated by SrtN and AcuC deacetylates (and thereby activates) AcsA PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed by glucose (4.5-fold) (CcpA) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 467 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 3357 PubMed

Biological materials

  • Mutant:
    • GP1212 (acsA::kan), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews


Original publications