LexA

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  • Description: transcriptional repressor of the SOS regulon

Gene name lexA
Synonyms dinR
Essential no
Product transcriptional repressor of the SOS regulon
Function regulation of DNA damage repair
Gene expression levels in SubtiExpress: lexA
Function and regulation of this protein in SubtiPathways:
lexA
MW, pI 22 kDa, 5.155
Gene length, protein length 615 bp, 205 aa
Immediate neighbours fosB, yneA
Sequences Protein DNA DNA_with_flanks
Genetic context
LexA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LexA expression.png




























Categories containing this gene/protein

DNA repair/ recombination, transcription factors and their control

This gene is a member of the following regulons

LexA regulon

The LexA regulon

The gene

Basic information

  • Locus tag: BSU17850

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Represses uvrB, dinB, dinC, recA genes and itself by binding to the 14 bp palindromic sequence 5'-CGAACNNNNGTTCG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. (according to Swiss-Prot)
  • Protein family: peptidase S24 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1JHH (from E. coli, S119A mutant, 33% identity, 52% similarity) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism: autorepression

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

M Butala, D Zgur-Bertok, S J W Busby
The bacterial LexA transcriptional repressor.
Cell Mol Life Sci: 2009, 66(1);82-93
[PubMed:18726173] [WorldCat.org] [DOI] (I p)


Original publications