LexA
Revision as of 13:50, 2 April 2014 by 134.76.38.147 (talk)
- Description: transcriptional repressor of the SOS regulon
Gene name | lexA |
Synonyms | dinR |
Essential | no |
Product | transcriptional repressor of the SOS regulon |
Function | regulation of DNA damage repair |
Gene expression levels in SubtiExpress: lexA | |
Function and regulation of this protein in SubtiPathways: lexA | |
MW, pI | 22 kDa, 5.155 |
Gene length, protein length | 615 bp, 205 aa |
Immediate neighbours | fosB, yneA |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
DNA repair/ recombination, transcription factors and their control
This gene is a member of the following regulons
The LexA regulon
The gene
Basic information
- Locus tag: BSU17850
Phenotypes of a mutant
Database entries
- BsubCyc: BSU17850
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Represses uvrB, dinB, dinC, recA genes and itself by binding to the 14 bp palindromic sequence 5'-CGAACNNNNGTTCG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. (according to Swiss-Prot)
- Protein family: peptidase S24 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU17850
- UniProt: P31080
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559]
[WorldCat.org]
[DOI]
(I p)
M Butala, D Zgur-Bertok, S J W Busby
The bacterial LexA transcriptional repressor.
Cell Mol Life Sci: 2009, 66(1);82-93
[PubMed:18726173]
[WorldCat.org]
[DOI]
(I p)
Original publications