PbpE

• Description: penicillin-binding protein PBP 4* (spore cortex)
  
  

Gene name	pbpE
Synonyms
Essential	no
Product	penicillin-binding protein PBP 4* (spore cortex)
Function	endopeptidase
Gene expression levels in SubtiExpress: pbpE
MW, pI	51 kDa, 4.835
Gene length, protein length	1353 bp, 451 aa
Immediate neighbours	racX, sacB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, cell wall degradation/ turnover, cell envelope stress proteins (controlled by SigM, V, W, X, Y), most abundant proteins

This gene is a member of the following regulons

SigW regulon

The gene

Basic information

• Locus tag: BSU34440

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: beta-lactamase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization: spore cortex

Database entries

• Structure: 3TG9 (from Bacillus halodurans, 27% identity, 59% similarity)

• UniProt: P32959

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pbpE-racX PubMed

• Expression browser: pbpE PubMed

• Sigma factor: SigW PubMed

• Regulation:
  • repressed during logrithmic growth (AbrB) PubMed, indirect regulation by AbrB PubMed
  • induced by cell wall stress (SigW) PubMed

• Regulatory mechanism:

• Additional information:
  • the mRNA is very stable (half-life > 15 min) PubMed
  • belongs to the 100 most abundant proteins PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: