PnbA

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  • Description: para-nitrobenzyl esterase

Gene name pnbA
Synonyms estB
Essential no
Product para-nitrobenzyl esterase
Function lipid degradation
Gene expression levels in SubtiExpress: pnbA
MW, pI 53 kDa, 4.776
Gene length, protein length 1467 bp, 489 aa
Immediate neighbours slrR, padC
Sequences Protein DNA DNA_with_flanks
Genetic context
PnbA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PnbA expression.png
























Categories containing this gene/protein

utilization of lipids, phosphoproteins

This gene is a member of the following regulons

AbrB regulon

The gene

Basic information

  • Locus tag: BSU34390

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Triacylglycerol + H2O = diacylglycerol + a carboxylate (according to Swiss-Prot)
  • Protein family: type-B carboxylesterase/lipase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Ser-189 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
    • repressed during logarithmic growth (AbrB) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
    • GP951 (pnbA::cat), available in Stülke lab
    • GP955 (slrR-pnbA::cat), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References